四川农业大学学报 ›› 1993, Vol. 11 ›› Issue (03): 339-345.doi: 10.16036/j.issn.1000-2650.1993.03.001

• 研究论文 •    下一篇

应用蛋白质和DNA片段电泳分析鉴定稻种上的黄色细菌

叶华智, 帅正彬, 伍光庆   

  1. 四川农业大学农学系, 雅安市 625014
  • 收稿日期:1992-12-25 出版日期:1993-09-30 发布日期:2017-04-22

IDENTIFICATION OF YELLOW-PIGMENTED BAVTERIA HABRING IN RICE SEEDS BY ELECTROPHORESIS ANALYSIS OF CELLU-IAR PROTEIN AND DNA REST. RICTION FRAGMENT

Ye Huazhi, et al   

  1. Sichuan Agricultural University, Yaan, Sichuan, China, Zip Code 625014
  • Received:1992-12-25 Online:1993-09-30 Published:2017-04-22

摘要: 本研究的目的是建立一种能简便、快速、准确鉴定水稻种子上寄藏的三类黄色细菌的方法。通过对三类菌株的菌体蛋白质和DNA限制性片段的电泳分析结果表明,三类细菌的蛋白质和DNA限制性片段电泳图谱明显不同,而在同一类群内不同菌株间的电泳图型则基本相同,因此能应用蛋白质和DNA限制性片段电泳分析于这些类群细菌的分类鉴定和亲缘关系分析,特别是菌体蛋白质是电泳分析具有简便、快速等优点。

关键词: 细菌, 水稻, 电泳, 凝胶电泳分析, 黄包细菌, 菌体蛋白质, DNA限制性片段

Abstract: The objective of the present study was to construct a rapid simple and reliable method for the diffrentiation of yellow-pigmen-ted bacteria habring in rice seeds. The callula protein and DNA restriction fragment from yellow-pigmented bacteria belonging to three groups identfied by morphlogical, physiological and biological features, and pathogenic tests were subjected to poly-acrymide vetical of agrose horizontal gel electrophoresis for their prontein and DNA restriction fragment patternsm, The results showed that the patterns of prontein and DNA frahment from diffrernt/groups were very distinct from each other, but the paterns of different strains in the same group were similar, so that the electro phoresis analysis can be used as a taxonomic criteria for diffenting them and the relationgship among.

Key words: BACTERIA, LOWLAND RICE, ELECTROPHORESIS, RICE BACTERIAL LEAF BLIGHT, BACTERIAL LEAF STREAK, PROTEIN, DNA RESTRICTION FRAGMENT

中图分类号: 

  • S511