四川农业大学学报 ›› 1991, Vol. 9 ›› Issue (01): 52-56.doi: 10.16036/j.issn.1000-2650.1991.01.008

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应用32P标记DNA探针检测伪狂犬病病毒的研究

郭万柱, 冯炳芳, 曹军, 徐重   

  1. 四川农业大学兽医学系, 雅安
  • 收稿日期:1990-08-29 出版日期:1991-03-31 发布日期:2017-04-22
  • 基金资助:
    国家自然科学基金资助

DETECTION OF PSEUDORABIES VIRUS BY 32p LABELED DNA PROBE

Guo Wanzhu, et al   

  1. Sichuan Agricultural University, Yaan Sichuan, China
  • Received:1990-08-29 Online:1991-03-31 Published:2017-04-22

摘要: 本文报道建立了一种以32P标记的PRV全基因组DNA和重组质粒PSAG1-19DNA作为探针,检测感染培养细胞中PRV-DNA的斑点杂交法。两种探针均能检测10Pg的PRVNDA,而与正常细胞抽提物无同源关系,具有较高的灵敏度和特异性。应用了一种快速、简便的PRV DNA抽提法,简化了样品制备的程序,提高了检测的可靠性。比较研究了杂交法中的一些重要条件,包括探针的选用,样品DNA的处埋,滤膜的选用等,为检测PRV提供了一种灵敏、可靠的方法。

关键词: 伪狂犬病病毒, 重组, 核酸, 检测, 传染病预防

Abstract: A dot blot hybridisation method was developed to detect pseudorabies. virus DNA in infected cultured cells. Five strains of pseudorabies virus were detected by 32P labeled pseudorabies virus DNA probe and recombinant plasmid DNA probe obtained by melecular cloning. Both probes could detect 10pg nucleic acid in the samples. The nucleic acid hybridisation method was sensitive and specific for diagnosing pscudorabies.

Key words: PSEUDORABIES VIRUS, RECOMBINATION, NUCLEIC ACIDS, DETECT/ON, INFECTIOUS

中图分类号: 

  • S858.292