四川农业大学学报

• 研究论文 • 上一篇    下一篇

暗孢节菱孢菌蛋白类毒素产生条件研究

朱天辉1,李姝江2   

  1. 1. 四川农业大学林学院
    2.
  • 收稿日期:2011-10-26 修回日期:2011-11-07 出版日期:2011-12-25 发布日期:2011-12-26
  • 通讯作者: 朱天辉

Production conditions of the protein toxin from Arthrinium phaeospermum

Zhu tianhui1,Li Shujiang2   

  1. 1.
    2. College of Forestry, Sichuan Agricultural University
  • Received:2011-10-26 Revised:2011-11-07 Online:2011-12-25 Published:2011-12-26
  • Contact: Zhu tianhui

摘要:

采用液体震荡培养结合硫酸铵分级沉淀法获取蛋白类粗毒素研究暗孢节菱孢菌(Arthrinium phaeospermum (corda)M.B.Ellis)产毒条件。经浸渍法生物活性检测并以杂交竹病情指数为评价标准,结果表明Fries培养液为最优产毒基质,病情指数达70.67%。不同培养时间、温度对该菌产毒量有较大的影响,12d左右是最佳产毒时间,25℃为产毒最适温度;该菌产毒对光照无特殊要求,产毒的较优溶氧量是介于120-160mL之间,在pH8.0时产毒较高。在优化条件下,该菌的产毒能力显著提高,杂交竹感病指数高达89.12%。

关键词: 杂交竹, 梢枯病, 毒素, 暗孢节菱孢, 生物活性

Abstract:

The toxin production conditions of Arthrinium phaeospermum were studied by acquiring the crude protein toxin with the methods of liquid shake culture and ammonium sulphate precipitation. According to impregnation method and disease index as the evaluation criteria, bioassay was tested in hybrid bamboo. The results showed that Fries culture medium was the optimal culture of toxin production, and the disease index reached 70.67%. Different culture time and temperature had greater effects on the amount of toxin production of this fungus, whose optimal time and temperature were 12d and 25℃. It had no special requirement for light, the satisfied dissolved oxygen content was between 120mL to 160mL, and the amount of toxin production was most at pH8.0. Under the optimized conditions, the ability of toxin production of this fungus had significantly increased, and the disease index of hybrid bamboo was up to 89.12%.

Key words: hybrid bamboo, blight, toxin, Arthrinium phaeospermum, biological activity