四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (03): 327-327.

• 研究论文 • 上一篇    下一篇

不同地理种源雷公藤的RAPD分析

李键1,范文洁2,陈昕2,谢安强2,洪伟2,吴承祯2   

  1. 1. 福建农林大学
    2.
  • 收稿日期:2011-05-24 修回日期:2011-07-11 出版日期:2011-09-30 发布日期:2011-10-13
  • 通讯作者: 李键
  • 基金资助:

    国家自然科学基金;教育部博士学科点专项基金资助项目;福建省科技重大专项

RAPD Analysis on Tripterygium wilfordii Hook.F in different Geological Provenances

  • Received:2011-05-24 Revised:2011-07-11 Online:2011-09-30 Published:2011-10-13
  • Contact: jian li

摘要:

应用随机引物扩增多态性DNA技术(RAPD),对25个不同地理种源的雷公藤进行了基因组DNA多态性分析,从105个随机引物中筛选出12个多态性较好的引物进行RAPD扩增,用UPGMA聚类法对扩增DNA片段数据构建系统发育树。研究结果表明:多态性条带百分率为91.14%,Shannon’s 信息指数I=0.4472,Nei’s基因多样性指数H =0.2973,观测等位基因数Na =1.9114,有效等位基因数Ne =1.5120。这表明25个种源具有较高的遗传多样性水平,因此收集不同种源以保存尽可能多的遗传多样对于雷公藤的良种选育是非常有必要的。

关键词: 雷公藤, RAPD;遗传多样性

Abstract:

Using RAPD technology, the DNA polymorphism analysis of genomes of Tripterygium wilfordii Hook.F from 25 different provenances was made. 12 primers which had good polymorphism chosen from the 105 Random Hexamers were used to RAPD amplification, and the data from the amplification of DNA fragments was used to construct the Phylogenetic Trees by UPGMA clustering methods. The results showed that the percentage of polymorphism bands is 91.14% of; The information index of Shannon’s was 0.4427; The Nei’s gene diversity index H was 0.2973; The number of the observed alleles was 1.9114, and the effective alleles’ number was 1.5120. These indexes all showed that the 25 tested groups had relatively high gene diversity. So samples of Tripterygium wilfordii Hook.F which should be collected from different populations for the reservation of the genetic diversity are very important to selecting improved variety.

Key words: Tripterygium wilfordii Hook.F, RAPD;gene diversity