›› 2018, Vol. 36 ›› Issue (04): 542-548.

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饲粮NDF水平对山羊营养物质表观消化率及瘤胃古菌结构与组成的影响研究

张雪娇,王立志,王之盛,薛白,彭全辉   

  1. 四川农业大学动物营养研究所
  • 收稿日期:2017-11-06 修回日期:2018-07-18 出版日期:2018-08-31 发布日期:2018-11-29
  • 通讯作者: 王立志 E-mail:wanglizhi08@aliyun.com
  • 基金资助:
    “十三五”国家重点研发计划项目“畜禽现代化饲养关键技术研发”

Effects of dietary NDF levels on apparent digestibility of nutrients and the composition of rumen archaea in goats

  • Received:2017-11-06 Revised:2018-07-18 Online:2018-08-31 Published:2018-11-29

摘要: 摘 要:(目的)本研究旨在采用代谢试验和高通量测序技术,研究饲粮中性洗涤纤维(NDF)水平对山羊营养物质表观消化率及瘤胃古菌结构与组成的影响。(方法)选用6只8月龄健康雄性山羊进行3×3拉丁方试验,依据饲粮NDF水平设低(LN组)、中(MN组)和高(HN组)NDF水平共三个处理组进行代谢试验。代谢试验共分三期进行,每期试验结束后采集瘤胃内容物,提取瘤胃微生物总DNA后,用古菌通用引物进行PCR扩增,扩增产物用Illumina平台进行高通量测序后进行生物信息分析。(结果)结果表明:1)处理组间山羊日粮DM、OM、CP表观消化率差异均不显著(P>0.05);LN组EE的表观消化率显著高于MN组和HN组(P<0.05),HN组NDF的表观消化率显著高于LN组和MN组(P< 0.05)。2)LN组古菌Chao1、Shannon和Observed_species指数均显著高于其它两组(P<0.05),其它两组间的差异不显著(P>0.05)。3)在门水平上,三个处理组间所有古菌的相对丰度差异均不显著(P>0.05),优势门均为广古菌门(Euryarchaeota);在属水平上,Methanobrevibacter,Candidatus_Methanomethylophilus和Candidatus_Methanoplasma均为三个处理组的优势属。LN组Methanobrevibacter的相对丰度极显著低于其它两组(P<0.01),但Candidatus_Methanomethylophilus和Candidatus_Methanoplasma的相对丰度显著高于其它两组(P<0.05),其余各菌属的相对丰度在三个处理组间差异均不显著(P>0.05)。(结论)研究结论:饲粮NDF水平在35%-45%之间变化时,显著影响山羊对日粮中EE、NDF的表观消化率,显著影响瘤胃Methanobrevibacter、Candidatus_Methanomethylophilus和Candidatus_Methanoplasma等古菌属的相对丰度。

关键词: 关键词:山羊, 营养物质表观消化率, 中性洗涤纤维, 古菌, 高通量测序

Abstract: Abstract: (Objective) This study was conducted to examine the effects of dietary NDF levels on apparent digestibility of nutrients and the composition of rumen archaea in goats using metabolic trial and high throughput sequencing technique. (Method) Six eight-month-old healthy male goats were chosen for the 3×3 Latin square experiments. Three experimental treatments (low dietary NDF level group (LN), middle dietary NDF level group (MN), and high dietary NDF level group (HN)) were set for metabolic trial. The whole experiment was divided into three phases, and rumen contents of each goat were collected after each phase. The total DNA of rumen microorganism in the rumen content was extracted. Primers specific for archaea were used to amplified the V3-V4 high variable regions of 16S rRNA gene. Bioinformatics analysis of PCR products was performed by high-throughput sequencing using Illumina platform. (Result) The results showed that: 1)There were no significant differences in the apparent digestibility of DM, OM and CP between the three groups (P>0.05) . The apparent digestibility of EE in LN group was significantly higher than that in MN group and HN group (P<0.05).The apparent digestibility of NDF in HN group was significantly higher than that in LN group and MN group (P<0.05). 2)The Chao1, Shannon and Observed species indices of LN group were significantly higher than those of the other two groups (P<0.05), but no significant difference between the other two groups (P>0.05). 3) At phylum level, the relative abundance of all taxonomy in the three treatment groups was not significant different (P>0.05), and the dominant phylum was Euryarchaeota.4)At genus level, Methanobrevibacter, Candidatus_Methanomethylophilus and Candidatus_Methanoplasma were the dominant genera of the three treatment groups. The relative abundance of Methanobrevibacter in LN group was remarkable significantly lower than that in the other two groups (P<0.01), but the relative abundance of Candidatus_Methanomethylophilus and Candidatus_Methanoplasma was significantly higher than that of the other two groups (P<0.05), these archaea were no significant difference between the other two groups. The other archaeal genera were no significant difference between the three treatments (P>0.05). Conclusion: When dietary NDF levels varied from 35% to 45%, the apparent digestibility of EE and NDF in diets was significantly affected in goats, and the relative abundance of major archaea such as Methanobrevibacter in rumen was significantly affected.

Key words: Key words: goat, nutrients apparent digestibility, neutral detergent fiber, archaea, high throughput sequencing