›› 2018, Vol. 36 ›› Issue (02): 261-266.

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EGCG通过NF-κB信号通路抑制感染ALV-J病毒的 DF-1细胞凋亡

张莹1,尹华东2,3,邬秀宏4,杨海滨1,钟应富1,袁林颖1,李中林1,徐泽1   

  1. 1. 重庆市农业科学院茶叶研究所
    2. 动物遗传育种研究所
    3. 四川农业大学
    4. 重庆市农业科学院
  • 收稿日期:2017-11-07 修回日期:2018-04-10 出版日期:2018-04-23 发布日期:2018-11-29
  • 通讯作者: 徐泽 E-mail:chongqingxuze@163.com
  • 基金资助:
    重庆市科委应用开发项目;重庆市科委基础与前沿项目;重庆市现代特色效益农业茶叶产业技术体系

Epigallocatechin Gallate (EGCG) Inhibited the Apoptosis in ALV-J- Infected DF-1 Cells by Inactivation of Nuclear Factor κB Pathway

  • Received:2017-11-07 Revised:2018-04-10 Online:2018-04-23 Published:2018-11-29

摘要: 【目的】评价表没食子儿茶素没食子酸酯(EGCG)是否具有抗J亚型禽白血病病毒(ALV-J)效应,为AVL-J防治药物的研发提供理论依据。【方法】在感染ALV-J病毒的DF-1细胞中分别添加浓度为0, 5, 10, 20和40 μg/mL的EGCG溶液后培养的0, 24, 48, 72和96h,对其细胞活性、细胞中env基因表达量,禽白血病p27抗原水平,NF-κB活性以及NF-κB p50和p65蛋白表达进行了测定。【结果】EGCG对感染ALV-J病毒后DF-1的保护作用具有剂量效应,10 μg/mL效果最好。在ALV-J侵染的DF-1细胞中添加10 μg/mL的EGCG溶液96 h后,可显著抑制由于ALV-J病毒导致的NF-κB亚基p50和p65跨膜转移,降低由于ALV-J病毒造成的细胞凋亡。【结论】EGCG通过抑制NF-κB信号的激活来提高DF-1细胞对ALV-J病毒的抵御能力,且具有显著的剂量效应,10 μg/mL为最佳添加浓度。

关键词: 表没食子儿茶素没食子酸酯, J亚型禽白血病病毒, NF-κB信号途径, DF-1细胞

Abstract: 【Objective】To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, and provide theoretical basis for the prevention and cure of ALV-J.【Method】DF-1 cells were treated with the EGCG concentrations of 0, 5, 10, 20 and 40 μg/mL, respectively. Then, the cell activity, the env mRNA expression, ALV p27 antigen level, NF-κB activity, the protein expression of NF-κB p50 and p65 were examined at the 0, 24, 48, 72 and 96 h after EGCG-treated.. 【Results】EGCG alleviated the ALV-J-induced apoptosis in a dose-dependent manner. High concentrations (20 and 40 μg/mL) inhibited the growth of DF-1 cell, and low concentration (5 μg/mL) did not suppress the ALV-J virus, so 10 μg/mL was the most appropriate concentration. After 96 h of incubation, 10 μg/mL EGCG improved the ALV-J-triggered suppression of the nuclear transcription factor system by enhancing cytoplasmic NF-κB p50/p65 expression and inhibiting nuclear NF-κB p50/p65 expression, resulting in decreased cell apoptosis. 【Conclusion】These results demonstrated that EGCG can increase the resistance ability to ALV-J in DF-1 cells with a dose-dependent manner via the NF-κB signaling pathway, and the optimum additive concentration was 10 μg/mL.

Key words: EGCG, Avian leucosis virus subgroup J (ALV-J), NF-κB pathway, DF-1 cell