四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (03): 299-303.

• 研究论文 •    下一篇

五份金荞麦核糖体基因内转录间隔区(ITS)序列分析

赵海霞   

  1. 四川农业大学
  • 收稿日期:2011-06-24 修回日期:2011-08-31 出版日期:2011-09-30 发布日期:2011-10-13
  • 通讯作者: 赵海霞

Sequences analysis of Internal Transcribed Spacer of Ribosome Gene among five Fagopyrum cymosum Samples

zhao haixia   

  1. sichuan agricultural university
  • Received:2011-06-24 Revised:2011-08-31 Online:2011-09-30 Published:2011-10-13
  • Contact: zhao haixia

摘要:

以五份金荞麦为材料,对其核糖体基因内转录间隔区(ITS)序列进行PCR扩增,均获得长约700 bp左右的特异性产物。测序结果表明,金荞麦样品中ITS序列在638 bp~797 bp之间。经软件Clustal X 2.0 和MEGA 4.0 分析结果表明,当空位作为缺失处理时,ITS区全序列包括488个保守位点,248个变异位点,67个简约信息位点,175个单个碱基变化位点。通过与GenBank已登录的金荞麦的ITS进行序列比对后发现,其同源度在86.8%~98.8%,其变异位点主要存在于ITS1,表现出丰富的遗传多样性。采用邻接法构建了系统进化树,结果表明,金荞麦主要分为两大类群,其遗传距离与其采集地有一定相关性。

关键词: 金荞麦, ITS, PCR, 序列分析

Abstract:

The genetic relationship among different Fagopyrum cymosum was revealed by analyzing the internal transcribed spacer (ITS) sequences of five F. cymosum samples. The sequencing results showed that there were about 700 bp including the whole ITS1, 5.8S and ITS2 regions. The further analysis indicated that ITS regions of five samples were 638 bp~797 bp in length. With lack of gap as treatment, the whole ITS sequence consisted of 488 conserved sites, 248 variable sites, 67 information sites and 175 singleton sites. The sequence similarity among thirteen F. cymosum samples varies from 86.8% to 98.8%, and the main variation occurs on ITS1 area. The data meant there was the abundant genetic diversity among different sources of F. cymosum. Phylogenetic tree was constructed by neighbour-joining method, which enriched genetic diversity of buckwheat at the molecular level. Analysis Results showed that F. cymosum was mainly divided into two groups, and the phylogenetic distance has some correlation with the locality of collection.

Key words: F. cymosum, ITS sequences, PCR, Sequence Analysis