四川农业大学学报 ›› 2010, Vol. 28 ›› Issue (03): 265-270.

• 研究论文 •    下一篇

Leymus duthiei及其变种Leymus duthiei var. longearistatus的 nrDNA ITS序列分析

凡星   

  1. 四川农业大学
  • 收稿日期:2010-07-20 修回日期:2010-08-30 出版日期:2010-09-30 发布日期:2010-09-30
  • 通讯作者: 凡星
  • 基金资助:

    国产赖草属物种的生物系统学研究及其分类处理;小麦族赖草属植物的分子系统发育研究

Sequence analysis of the ITS region of nuclear ribosomal DNA (nrDNA) in Leymus duthiei and Leymus duthiei var. longearistatus

xing 0fan   

  1. Sichuan agricultural university
  • Received:2010-07-20 Revised:2010-08-30 Online:2010-09-30 Published:2010-09-30
  • Contact: xing 0fan

摘要:

本研究对Leymus duthiei及其变种Leymus duthiei var. longearistatus的nrDNA ITS进行多克隆测序,探讨ITS序列在它们个体内的多样性和个体间的遗传分化。序列多态性、基因谱系和rRNA基因二级结构分析表明:(1)由于致同进化的作用,ITS序列在L. duthiei和L. duthiei var. longearistatus中可能朝Ns基因组一方发生偏向;(2)致同进化的驱动力可能促使不同的ITS拷贝序列在L. duthiei var. longearistatus中比在L. duthiei中更趋于一致;(3)ITS序列在L. duthiei和L. duthiei var. longearistatus中的遗传分化可能与它们的地理隔离有关。

关键词: Leymus duthiei, Leymus duthiei var. longearistatus, nrDNA ITS, 致同进化

Abstract:

Sequencing of multiple clones of ribosomal ITS sequences in Leymus duthiei and Leymus duthiei var. longearistatus were performed to estimate ITS polymorphism within and between individual. Sequence diversity patterns, ITS RNA secondary structure and genealogical analysis suggested that (1) due to concerted evolution, ribosomal ITS sequences in L. duthiei and L. duthiei var. longearistatus may be biased towards the Ns genome donor; (2) concerted evolution might promote the ribosomal ITS sequences within L. duthiei var. longearistatus more homogenized than that within L. duthiei; (3) ITS sequence divergence between L. duthiei and L. duthiei var. longearistatus may be responsible for their geographic isolation.

Key words: Leymus duthiei, Leymus duthiei var. longearistatus, nrDNA ITS, concerted evolution