四川农业大学学报 ›› 2014, Vol. 32 ›› Issue (04): 398-407,425.doi: 10.3969/j.issn.1000-2650.2014.04.008

• • 上一篇    下一篇

葡萄柚(Citrus paradisi Macf.)实生苗根段的离体培养及植株再生研究

陶燕蓝1, 蓝增全1, 吴田2   

  1. 1. 西南林业大学环境科学与工程学院, 昆明 650224;
    2. 西南林业大学园林学院, 昆明 650224
  • 收稿日期:2014-05-26 出版日期:2014-12-31 发布日期:2017-02-28
  • 通讯作者: 吴田,副教授,博士,主要从事植物生物技术方面的研究,E-mail:461257271@qq.com;蓝增全,教授,硕士,主要从事森林生态学及植物学方面研究,E-mail:kmlanzengquan@qq.com。 E-mail:461257271@qq.com;kmlanzengquan@qq.com
  • 作者简介:陶燕蓝,硕士研究生。
  • 基金资助:
    云南省教育厅重点项目(2014C006Z);国家林业局948项目(2011-4-45)

Culture in vitro and Plant Regeneration from Roots of Citrus paradisi Macf. Seedlings

TAO Yan-lan1, LAN Zeng-quan1, WU Tian2   

  1. 1. College of Environmental Science and Engineering, Southwest Forestry University, Kunming 650224, China;
    2. College of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, China
  • Received:2014-05-26 Online:2014-12-31 Published:2017-02-28

摘要: [目的] 建立葡萄柚(Citrus paradisi Macf.)离体再生体系,为后续遗传转化体系的建立奠定基础。[方法] 以10个葡萄柚品种种子萌发的无菌苗根段为材料,在附加不同植物激素种类及浓度的培养基上进行离体培养,并获得再生植株。[结果] 在MS+0.5 mg/L 6-BA+0.5 mg/L NAA培养基上培养可以诱导出愈伤组织,其中"帕利斯"的诱导率最高,达87.95%;在培养基MS+2.0 mg/L 6-BA+0.5 mg/L NAA可分化出丛生芽,其中"星路比"分化率最高为37.84%;分化出的丛生芽在MS+2.0 mg/L 6-BA+0.2 mg/L NAA培养基上可以进行增殖培养;在1/2MS+1.0 mg/L IBA上可诱导出不定根,形成完整再生植株。炼苗移栽成活率均可达75.47%~88.40%。[结论] 通过对10个葡萄柚品种根段离体再生培养,建立了6个品种的离体再生体系。

关键词: 葡萄柚, 离体培养, 根, 不定芽

Abstract: [Objective] The objective of the study was to establish regeneration system of C. paradisi in vitro and to provide useful references for the application of genetic transformation technique. [Method] The roots of aseptic seedlings germinated from the seeds of 10 grapefruit varieties were cultured in vitro to obtain regeneration plants in the medium with different hormones and concentrations. [Results] The callus were observed in the medium MS+0.5 mg/L 6-BA+0.5 mg/L NAA and Perlist had the highest callus induction frequency (87.95%).The differentiation of adventitious buds was observed in the medium MS+2.0 mg/L 6-BA+0.5 mg/L NAA and Star Ruby had the largest differentiation rate of adventitious buds (37.84%).The regeneration buds were multiplied in the medium MS+2.0 mg/L 6-BA+0.2 mg/L NAA. The adventitious roots were induced in the medium 1/2 MS+1.0 mg/L IBA and the regeneration plants finally were obtained.The survival rates of regenerative plant ranged from 75.47% to 88.40%. [Conclusion] The regeneration systems of 6grapefruit varieties were established based on the study.

Key words: Citrus paradisi Macf., culture in vitro, root, adventitious bud

中图分类号: 

  • Q943.1