四川农业大学学报 ›› 2014, Vol. 32 ›› Issue (02): 205-212.doi: 10.3969/j.issn.1000-2650.2014.02.014

• • 上一篇    下一篇

LPS对兔下丘脑、输卵管上FSHR和LHR表达的影响

张谊1, 兰瑞霞2, 陈晨2, 刘淑君2, 张明2   

  1. 1. 西昌学院动物科学学院, 四川 西昌 615013;
    2. 四川农业大学动物科技学院, 四川 雅安 625014
  • 收稿日期:2014-03-27 出版日期:2014-06-30 发布日期:2017-02-28
  • 通讯作者: 张明,教授,硕士生导师,主要研究方向:动物繁殖生物技术与胚胎生物工程,E-mail:zhm3000@163.com。 E-mail:zhm3000@163.com
  • 作者简介:张谊,副教授,主要从事动物遗传育种与繁殖方面研究,E-mail:zhangyi707@163.com。
  • 基金资助:
    国家人力资源部和社会保障部留学人员科技活动项目

Effect of LPS on the Expression of FSHR and LHR in Hypothalamus and Oviduct of Rabbit

ZHANG Yi1, LAN Rui-xia2, CHEN Chen2, LIU Shu-j un2, ZHANG Ming2   

  1. 1. School of Animal Science, Xichang College, Xichang 615013, Sichuan, China;
    2. College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2014-03-27 Online:2014-06-30 Published:2017-02-28

摘要: [目的] 检测促卵泡素受体(FSHR)和促黄体素受体(LHR)蛋白在下丘脑、输卵管的分布,以及LPS诱导后母兔下丘脑、输卵管上FSHR和LHR的表达变化。[方法] 36只雌性新西兰白兔随机分为9组(n=4),一组不注射作为0 h组,LPS处理组按0.5 mg/kg体重耳缘静脉注射LPS,对照组注射等量的生理盐水,在注射后0、1.5、3、6和12 h后处死,收集下丘脑和输卵管,采用荧光定量PCR和免疫组化在转录水平和蛋白水平分析FSHR和LHR的表达。[结果] 结果显示:FSHR和LHR主要定位于输卵管黏膜层和肌层,在间质层未检测到FSHR和LHR;LPS诱导下,LPS不影响FSHR和LHR mRNA在下丘脑的转录,但下调FSHR和LHR mRNA在输卵管的表达。蛋白水平上,LPS改变了FSHR在下丘脑和LHR在输卵管黏膜层的表达模式,对LHR在下丘脑和输卵管肌层有下调作用,对FSHR在输卵管黏膜层和肌层有上调作用。[结论] LPS影响FSHR和LHR蛋白在下丘脑的表达,表明免疫-繁殖系统可以在下丘脑发生相互作用,进而影响动物生殖。

关键词: 兔, LPS, FSHR, LHR, 下丘脑, 输卵管

Abstract: [Objective] The aim of this study was to locate Follicle-stimulating hormone receptor (FSHR) and Luteinizing hormone receptor (LHR)LHR in hypothalamus oviduct,and determine whether Lipopolysaccharide (LPS)impact on the expression of FSHR and LHR in hypothalamus and oviduct. [Method] 36mature New Zealand female rabbits were divided into 9 groups (n=4),one group non-treatment and worked as 0 h group,LPS group injected with LPS (0.5 mg/kg)or saline (control).The hypothalamus and oviduct were collected at 0,1.5,3,6 and 12 h after injection.The FSHR and LHR were located in oviduct by immunohistochemistry,and expression level of FSHR and LHR were analyzed by real-time PCR and immunohistochemistry. [Results] The results shown:FSHR and LHR were located in epithelium and muscle layer of oviduct,and they were not detected in stromal layer.The transcription of FSHR and LHR in hypothalamus were not influenced by LPS,while down-regulated in oviduct.Immunohistochemistry results shown:the expression pattern of FSHR in hypothalamus and LHR in epithelium layer of oviduct may be modulated by LPS,the expression of LHR in hypothalamus and muscle layer of oviduct was down-regulated by LPS,and the expression of FSHR in epithelium and muscle layer of ovi-duct was up-regulated by LPS. [Conclusion] The fact that LPS modulates FSHR and LHR gene expression in hypothalamus indicates that this brain structure could be a place of the immune-reproductive interference and supports the thesis that immune challenges modulate the activity of animal reproduction.

Key words: rabbit, Lipopolysaccharide, Follicle-stimulating hormone receptor, Luteinizing hormone receptor, hypothalamus, oviduct

中图分类号: 

  • S829.1