四川农业大学学报 ›› 2014, Vol. 32 ›› Issue (01): 76-81.doi: 10.3969/j.issn.1000-2650.2014.01.013

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家兔PPARγ基因编码区多态性及其与生长性状的关联分析

李春梅, 贾先波, 陈仕毅, 王杰, 赖松家   

  1. 四川农业大学动物遗传育种研究所, 四川 温江 611130
  • 收稿日期:2014-01-13 出版日期:2014-03-31 发布日期:2017-02-28
  • 通讯作者: 赖松家,教授,生物技术与兔遗传育种,E-mail:laisj5794@gmail.com。 E-mail:laisj5794@gmail.com
  • 作者简介:李春梅,硕士研究生。
  • 基金资助:
    四川省"十二五"科技支撑项目(编号:2011N20099-4);国家现代农业技术体系项目(编号:CARS-44-A-2)

Association between Polymorphisms of the Coding Region of PPARγ Gene and the Growth Traits in Rabbits

LI Chun-mei, JIA Xian-bo, CHEN Shi-yi, WANG Jie, LAI Song-jia   

  1. Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Weniiang 611130, Sichuan, China
  • Received:2014-01-13 Online:2014-03-31 Published:2017-02-28

摘要: [目的] 探究PPARγ基因编码区多态性与家兔生长性状的关联性。[方法] 采用直接测序检测PPARγ基因编码区变异位点,利用PCR-RFLP技术对352只家兔(新西兰271只,艾格81只)PPARγ基因的候选SNP进行基因分型。[结果] 在家兔PPARγ基因编码区只检测到一个同义突变位点c.207A>C,CC基因型个体70日龄体重显著高于AC基因型个体(P<0.05)。且CC基因型个体35到70日龄的平均日增重极显著高于AC、AA基因型个体(P<0.01)。[结论] PPARγ可作为影响家兔生长性状的候选基因。

关键词: PPARγ基因, 多态性, PCR-RFLP, 生长性状, 家兔

Abstract: [Objective] The aim of this study was to investigate the association between the Polymorphisms of coding region of peroxisome proliferator-activated receptor gamma (PPARγ)gene and growth traits. [Method] The polymorphisms in coding region of PPARγwere detected by direct sequencing and the genotyping for candidate SNP was performed on 352rabbits including 271 New Zealand White rabbits and 81 Etam rabbits. [Results] Only one single nucleotide polymorphism,c.207A>C was detected in the coding region of PPARγ.The body weight at 70 days of CC genotype individuals was significantly higher than that of AC genotype individuals (P<0.05). In addition,the average daily gain (ADG)from 35 days to 70 days of genotype CC was highly significantly different from genotypes AA (P<0.01) and AC (P<0.01). [Conclusion] PPARγ could be a candidate gene affecting the growth traits in rabbits.

Key words: PPARγgene, polymorphism, PCR-RFLP, growth traits, rabbits

中图分类号: 

  • S829.1