四川农业大学学报 ›› 2014, Vol. 32 ›› Issue (01): 59-62.doi: 10.3969/j.issn.1000-2650.2014.01.010

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瓜蒌瓤提取物对酪氨酸酶抑制作用的研究

徐翠翠1, 孔浩1, 郭庆梅1, 周凤琴1, 王晓2   

  1. 1. 山东中医药大学, 济南 250355;
    2. 山东省分析测试中心, 济南 250014
  • 收稿日期:2013-12-02 出版日期:2014-03-31 发布日期:2017-02-28
  • 通讯作者: 郭庆梅,教授,博士生导师,从事中药质量控制与资源研究方面的工作,E-mail:qmguo@sina.com;周凤琴,教授,博士生导师,从事中药质量控制与资源研究方面的工作,E-mail:zfq_sdzy@163.com。 E-mail:qmguo@sina.com;zfq_sdzy@163.com
  • 作者简介:徐翠翠,硕士生。
  • 基金资助:
    山东省科技发展计划项目(编号:2011GSF11904);国家科技支撑计划(编号:2011BAI06B06)

Inhibiting Effect of Extract from Fruit Pulp of Trichosanthes kirilowii Maxim. on Tyrosinase Activity

XU Cui-cui1, KONG Hao1, GUO Qing-mei1, ZHQU Feng-qin1, WANG Xiao2   

  1. 1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
    2. Shandong Analysis and Test Center, Jinan 250014, China
  • Received:2013-12-02 Online:2014-03-31 Published:2017-02-28

摘要: [目的] 探讨瓜蒌瓤提取物对酪氨酸酶的抑制作用。[方法] 考察了提取溶剂、L-酪氨酸量、酪氨酸酶的量、反应时间等因素对酪氨酸酶抑制率的影响。[结果] 当加入50 μL浓度为0.362 mg/mL的L-酪氨酸,50 μL活力为327 U/mL的酪氨酸酶,反应5 min时,瓜蒌瓤提取物对酪氨酸酶有较明显的抑制作用。[结论] 瓜蒌瓤提取物对酪氨酸酶有明显的抑制作用,可作为化妆品的天然美白添加剂。

关键词: 瓜蒌瓤, L-酪氨酸, 酪氨酸酶, 抑制作用

Abstract: [Objective] The aim of the study was to explore the inhibiting effect of extract from fruit pulp of T. kirilowii on tyrosinase activity. [Method] In this study,microculture plate reader was used to investigate the effects of extraction solvent,L-tyrosine volume,the amount of tyrosinase and reaction time on tyrosinase activity. [Results] When 50 μL L-tyrosine with 0.362 mg/mL and 50 μL tyrosinase with 327 U/mL were added,and the reaction time was 5 min,the inhibiting effect of extract from fruit pulp of T. kirilowii on tyrosinase activity were the most significant. [Conclusion] The extract from fruit pulp of T. kirilowii had significant inhibiting effects on tyrosinase activity.Therefore,it can be used as a natural whitening cosmetic additive.

Key words: fruit pulp of Trichosanthes kirilowii Maxim., L-tyrosine, tyrosinase, inhibition

中图分类号: 

  • Q946