四川农业大学学报 ›› 2013, Vol. 31 ›› Issue (04): 423-426.doi: 10.3969/j.issn.1000-2650.2013.04.012

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GARS-AIRS-GART基因多态性及其与肉质性状的关联性研究

张增荣1, 杜华锐1, 李晴云1, 杨朝武1, 邱莫寒1, 李雯2, 刘岚2, 辅宏璞3, 蒋小松1   

  1. 1. 四川省畜牧科学研究院, 成都 610066;
    2. 四川大恒家禽育种有限公司, 成都 610066;
    3. 西南民族大学生命科学与技术学院, 成都 610041
  • 收稿日期:2013-09-11 出版日期:2013-12-31 发布日期:2017-02-28
  • 作者简介:张增荣,助理研究员,E-mail:Zhangzengrong2004@163.com。
  • 基金资助:
    四川省财政基础科研项目;四川省畜牧科学研究院基本科研项目(编号:SASA2010A02)

Association of the GARS-AIRS-GART Gene Polymorphisms and Meat Quality Traits in Chickens

ZHAN Zeng-rong1, DU Hua-rui1, LI qing-yun1, YAN Chao-wu1, FU Hong-pu1, QIU Mo-han2, LI Wen2, LIU Lan3, JIANG Xiao-song1   

  1. 1. Sichuan Animal Science Academy, Chengdu 610066, China;
    2. Sichuan Daheng poultry breeding company, Chengdu 610066, China;
    3. Southwest University for Nationalities, Chengdu 610041, China
  • Received:2013-09-11 Online:2013-12-31 Published:2017-02-28
  • Contact: 蒋小松,研究员,主要从事家禽遗传育种研究工作,E-mail:xsjiang@sasa.cn。 E-mail:xsjiang@sasa.cn

摘要: [目的] 探讨GARS-AIRS-GART基因对鸡肉质风味性状的影响。[方法] 以大恒优质肉鸡品系为研究对象,利用PCR-SSCP和测序方法对GARS-AIRS-GART基因的多态性进行研究。[结果] 研究结果显示:GARS-AIRSGART基因外显子4有1个SNP位点位于6545处(A→C突变),该位点为错义突变并导致氨基酸序列第173位点由天冬氨酸(Asp)突变为谷氨酸(Glu)。GARS-AIRS-GART-6545SNP位点对鲜味氨基酸含量和肌苷酸含量有显著影响(P<0.05),NN型表现为高肌苷酸含量。[结论] 由此推测GARS-AIRS-GART基因可能是影响鸡肉质性状的主效基因或与控制这些性状的QTL连锁。

关键词: 鸡, GARS-AIRS-GART基因, SNPs, 肉质性状

Abstract: [Objective] The aim of this study was to detect the impact of the glycinamide ribonucleotide synthetase (GARS),aminoimidazole ribonucleotide synthetase (AIRS) and glycinamide ribonucleotide formyltransferase (GART) enzymatic (GARS-AIRS-GART) gene on chicken meat flavor traits.[Method] PCR-SSCP and sequencing technology were employed in this study to detecte the polymorphisms of the GARS-AIRS-GARTgene in Dahen chickens.[Results] One single nucleotide polymorphism was detected in GARS-AIRS-GART gene exon 4at site 6545 (A→C), which,as a missense mutation leading to the amino acid changing from Asp to Glu.Significant association has been found between GARS-AIRS-GART-6545SNP and flavor amino acid content as well as IMP (P<0.05).Among the genotypes,NN had higher IMP content.[Conclusion] GARS-AIRS-GARTgene may play an importance part in affecting chicken meat quality traits or linked with the related QTLs.

Key words: chicken, GARS-AIRS-GARTgene, SNPs, meat quality traits

中图分类号: 

  • S831