四川农业大学学报 ›› 2013, Vol. 31 ›› Issue (01): 93-98.doi: 10.3969/j.issn.1000-2650.2013.01.018

• 研究论文 • 上一篇    下一篇

响应面优化超声辅助醇碱法提取厚朴总酚的工艺条件

徐洲1, 李旭1, 郭军伟2, 丁春邦1   

  1. 1. 四川农业大学生命科学与理学院, 四川 雅安 625014;
    2. 雅安太时生物科技有限公司, 四川 雅安 625000
  • 收稿日期:2012-11-26 出版日期:2013-03-31 发布日期:2017-02-28
  • 通讯作者: 丁春邦,博导,教授,从事植物资源评价与利用研究,E-mail:dcb@sicau.edu.cn。 E-mail:dcb@sicau.edu.cn
  • 作者简介:徐洲,本科在读。
  • 基金资助:
    四川省科技厅科技支撑计划项目(2011SZ0283)

Response Surface Optimization for Ultrasonic Ethanol-alkali Extraction of Total Phenols of Magnolia of ficinalis var. biloba

XU Zhou1, LI Xu1, GUO Jun-wei2, DING Chun-bang1   

  1. 1. College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Yaan Times Biotech Co., Ltd, Yaan 625000, Sichuan, China
  • Received:2012-11-26 Online:2013-03-31 Published:2017-02-28

摘要: [目的] 优化醇碱法提取厚朴总酚(和厚朴酚、厚朴酚)的工艺条件。[方法] 以和厚朴酚、厚朴酚、厚朴总酚得率为指标,考察乙醇浓度、碱浓度、料液比、超声时间对提取效率的影响,采用响应面法分析各因素的影响程度和各因素间的相互作用。[结果] 建立的二次回归方程与试验数据拟合度高、预测性好,和厚朴酚、厚朴酚、厚朴总酚的R2分别为0.9402、0.9626、0.9606。经响应面分析,在超声强度150W下,提取厚朴的最佳条件为乙醇浓度56%、碱浓度0.8%、料液比1:36、超声时间29min,在此条件下和厚朴酚、厚朴酚、厚朴总酚得率分别为1.95、9.80、11.75mg/g。[结论] 所建立的回归方程对厚朴提取条件的优化是可行的,优选出的最佳工艺具有提取得率高、时间短、耗能少等优点。

关键词: 醇碱法, 响应面分析, 厚朴酚, 和厚朴酚

Abstract: [Objective] Optimize the extraction condition of highest yields of total phenols (magnolol,honokiol) for cortex Magnolia of ficinalis var. biloba,using the response surface analysis.[Method] The extraction factors of the concentration of ethanol,concentration of alkali,solid-to-liquid and periods of ultrasound-assisted extraction were employed.[Results] The second-order quadratic regression equation fitted the experimental data well with regard to magnolol (R2=0.940 2),honokiol (R2=0.962 6) and total phenols (R2=0.960 6),respectively.Under the condition of ultrasonic power 150 W,the optimum extraction conditions for total phenols were ethanol 56%,alkali 0.8%,solid-to-liquid 1:36,period of extraction 29 min,where herein yields of magnolol were 1.95 mg/g,honokiol were 9.80 mg/g,total phenols were 11.75 mg/g.[Conclusion] The predicted values got consistent with experimental values.Ethanol-alkali extraction of total phenols dominated in time,power and efficiency,comparing with ethanol and alkali extraction.

Key words: ethanol-alkali extraction, response surface analysis, magnolol, honokiol

中图分类号: 

  • R284.2