四川农业大学学报 ›› 2012, Vol. 30 ›› Issue (04): 463-467.doi: 10.3969/j.issn.1000-2650.2012.04.017

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复方中草药对鲫鱼免疫力的影响

盛竹梅1, 马志宏2, 黄文1, 杨华莲1, 马立鸣1, 申惠卿3, 曹洁1, 曹爱英1   

  1. 1. 北京市水产技术推广站, 北京 100021;
    2. 北京市水产科学研究所, 北京 100068;
    3. 延庆县水产服务中心, 北京 102100
  • 收稿日期:2012-08-30 出版日期:2012-12-31 发布日期:2017-02-28
  • 作者简介:盛竹梅,高级工程师,主要从事水产养殖与水产病害防控研究,E-mail:zhumeisheng@gmail.com。
  • 基金资助:
    北京市财政局项目(PXM2009-036237-073415)

Effect of Chinese Herbal Compounds on Immunity of Crucian

SHENG Zhu-mei1, MA Zhi-hong2, HUANG Wen1, YANG Hua-lian1, MA Li-ming1, SHEN Hui-qing3, CAO Jie1, CAO Ai-ying1   

  1. 1. Beijing Aquatic Product Technology Promotion Department, Beijing 100021, China;
    2. Beijing Fisheries Research Institute, Beijing 100068, China;
    3. Beijing Yanqing Fisheries Service Center, Beijing 102100, China
  • Received:2012-08-30 Online:2012-12-31 Published:2017-02-28
  • Contact: 马志宏,高级工程师,主要从事水产养殖与水产养殖动物病害研究,E-mail:mzh255@sohu.com。 E-mail:mzh255@sohu.com

摘要: [目的] 研究中草药对鲫鱼免疫力的影响。[方法] 将麻黄、苦参、黄芩、五倍子、紫苏、石榴皮6余味中草药均匀粉碎后制成复方制剂,熬制成汤剂,用生理盐水配制成质量分数为0(对照组)、0.5%(A组)、2.0%(B组)、4.0%(C组)的药液后对鲫鱼采取腹腔注射的方式给药。在试验的第7、14、21、28天分批采取血样,检测其免疫指标的变化。[结果] 给药后第7天,实验组血清溶菌酶活性均明显高于对照组(P<0.05),而且浓度越大,活性越高;实验组的血清杀菌活性均明显高于对照组(P<0.05),其中4.0%的C组效果最好,另外两组效果相当;在第7天,A组NBT阳性细胞数和对照组无显著差异性,B组和C组显著高于对照组,从第14天起,实验组均显著高于对照组,且各实验组间有显著差异;在血液白细胞吞噬活性检测中,从第7天起,对照组均显著高于对照组,各实验组间存在显著性,而在第28天,各实验组间差异不明显。经过注射中草药液后,各实验组间鲫血液皮质醇水平及脾脏指数的变化差异不显著。[结论] 该复方中草药制剂能有效地提高鲫鱼非特异免疫力。

关键词: 中草药, 免疫力, 鲫鱼, 腹注

Abstract: [Objective] Our study aimed at studying effects of Chinese herbal medicine on immunity of Crucian. [Method] More than six Chinese herbal medicines(ephedra, kuh-seng, astragalus mongholicus. etc) were grinded evenly then made into compound preparation by being boiled into medicinal juice after which the juices divided into 0(control group), 0.5%(A group), 2.0%(B group), 4.0%(C group) by percentage of mass with normal saline and then injected into crucian carps from intraperitoneal cavity. The blood collected on the 7th, the 14th, the 21st, the 28th day during the experiments for examining their immune indexs. [Results] the serum lysozyme activity in experimental groups was significantly higher than control group(P<0.05) since the first week, in addition, the higher the concentration was, its activity was higher accordingly;the serum bactericidal activity in experimental groups was significantly higher than control group(P<0.05), especially significant in group C, and the other two groups were at the same level;On the 7th day, there was no significant difference between the number of NBT positive cells in group A and control group, but significant in group B and group C between control group;and since the 7th day, all the experimental groups were significantly higher than control group, significance lied between all of them;On the 28th day, difference turned insignificant. After the injection of herbal medicine, the serum cortisol level among crucian carps made some difference, the mass index of spleen was relatively increased, but the effects were not significant. [Conclusion] The Chinese herbal medicine can improve the crucian carp's non-specific immunity effectiveness.

Key words: Chinese herbal medicine, immunity, Carassius auratus, intraperitoneal injection

中图分类号: 

  • S942.5