四川农业大学学报 ›› 2012, Vol. 30 ›› Issue (03): 267-271.doi: 10.3969/j.issn.1000-2650.2012.03.001

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普通小麦-华山新麦草人工合成双二倍体AFLP标记多态性分析

袁璟亚, 康厚扬, 王益, 钟美玉, 周永红   

  1. 四川农业大学小麦研究所, 四川 温江 611130
  • 收稿日期:2012-05-14 出版日期:2012-09-30 发布日期:2017-02-28
  • 基金资助:
    国家自然科学基金项目(31101151);国家科技计划(863)项目(2011AA100103)

Polymorphism Analysis of Wheat-Psathyrostachys huashanica Amphiploids with AFLP

YUAN Jing-ya, KANG Hou-yang, WANG Yi, ZHONG Mei-yu, ZHOU Yong-hong   

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China
  • Received:2012-05-14 Online:2012-09-30 Published:2017-02-28
  • Contact: 周永红,博士,教授,主要研究方向:植物系统与进化,E-mail:zhouyh@sicau.edu.com。 E-mail:zhouyh@sicau.edu.com

摘要: [目的] 从分子水平上了解普通小麦-华山新麦草双二倍体(PHW-SA)合成过程中遗传变异情况。[方法] 用195对引物对PHW-SA及其亲本普通小麦(J-11)和华山新麦草进行AFLP标记分析。[结果] J-11、华山新麦草和PHW-SA分别扩增出标记5136、3953和5637条,分别占总标记(17726条)的29.0%、22.3%和31.8%。81.8%的J-11标记和49.6%的华山新麦草标记存在于PHW-SA中。其中J-11与PHW-SA特有相同标记2484条,分别占各自标记的48.4%和44.1%;华山新麦草与PHW-SA特有相同标记246条,分别占各自标记的6.2%和4.4%;PHW-SA特有标记232条,缺失双亲共有标记66条,分别占PHW-SA总标记的4.1%和1.2%;3种材料共有相同标记1715条,分别占J-11、华山新麦草和PHW-SA总标记的33.4%、43.4%和30.4%。[结论] PHW-SA在合成过程中基因组发生了较大的遗传变异。此研究结果为PHW-SA在小麦遗传育种中的应用提供了基础理论依据。

关键词: 华山新麦草, AFLP标记, 遗传变异

Abstract: [Objective] The purpose of this study was to analyze the genetic variation of PHW-SA. [Method] 195 pairs of AFLP primers were used to amplify PHW-SA and its parents J-11(common wheat) and Psathyrostachys huashanica. [Results] 5 136, 3 953 and 5 637 markers were amplified in J-11, Psathyrostachys huashanica and PHW-SA, respectively, accounted for 29.0%, 22.3% and 31.8% of the total(17 726 banding), respectively;81.8% markers of J-11 and 49.6% markers of Psathyrostachys huashanica were amplified in PHW-SA;there were 2 484 co-markers between PHW-SA and J-11, which accounted for 48.4% and 44.1% of themselves, respectively;similarly, 246 co-markers existed between(6.2%) PHW-SA and(4.4%) Psathyrostachys huashanica;232 new markers which did not exist in its parents were amplified in PHW-SA and 66 markers of its parents could not be amplified in PHW-SA;there were 1 715 co-markers in all 3 materials, accounted for 33.4%, 43.4% and 30.4% of its total markers of J-11, Psathyrostachys huashanica and PHW-SA, respectively. [Conclusion] All evidence suggest that great genetic variation was occurred in the genome of PHW-SA when synthesized, and provide the theoretical information for wheat breeding in future.

Key words: Psathyrostachys huashanica, AFLP marker, hereditary variation

中图分类号: 

  • S512.1