四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (04): 540-543.doi: 10.3969/j.issn.1000-2650.2011.04.019

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猪载脂蛋白E基因的组织表达谱分析

李仕新1,2, 高萍2, 李加琪2, 陈赞谋2, 臧莹安1   

  1. 1. 仲恺农业工程学院生命科学学院, 广州 510225;
    2. 广东省农业动物基因组学与分子育种重点实验室, 华南农业大学动物科学学院, 广州 510642
  • 收稿日期:2011-11-14 出版日期:2011-12-31 发布日期:2017-04-12
  • 通讯作者: 李加琪,博士,教授,研究方向:动物遗传育种与繁殖,E-mail:jqli@scau.edu.cn。 E-mail:jqli@scau.edu.cn
  • 作者简介:李仕新,博士,研究方向:动物遗传育种与繁殖,E-mail:lishixin6618@163.com。
  • 基金资助:
    国家自然科学基金(30771545);广东省自然科学基金博士启动项目(10451022501005709)

Analysis of Tissue-specific Expression Profile of Porcine APOE

LI Shi-xin1,2, GAO Ping2, LI Jia-qi2, CHEN Zan-mou2, ZANG Ying-an1   

  1. 1. College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    2. Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
  • Received:2011-11-14 Online:2011-12-31 Published:2017-04-12

摘要: 载脂蛋白E在胆固醇和其他脂质的代谢中起着至关重要的作用,对猪APOE基因的组织表达谱分析是研究该基因功能的重要基础工作。研究采用RT-PCR的方法对APOE在长白猪的13个组织中的表达进行了研究,结果显示APOE基因主要在肝和脑组织表达,其他外周组织如脾、肺、肾、大肠、小肠也有不同程度的表达,而在心、胃、背肌和淋巴中几乎不表达。

关键词: 猪, 载脂蛋白E, 组织表达谱

Abstract: Apolipoprotein E plays an important role in cholesterol and other lipid metabolism and studying on tissue expression files of porcine APOE gene was an important basis for researching functions of the gene.In this study, RT-PCR method was used to study on expression of APOE in thirteen tissues of Landrace and the results showed that APOE gene was mainly expressed in the liver and brain tissue, and there were different expression in other peripheral tissues such as spleen, lung, kidney, large intestine, small intestine, whereas almost no expression in heart, stomach, M. longissimus and lymph in pig.

Key words: pig, APOE, tissue-specific expression profile

中图分类号: 

  • S828.212