四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (03): 402-406.doi: 10.3969/j.issn.1000-2650.2011.03.019

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高大毛霉液体深层培养研究

龙菊1, 吴拥军2, 何映霞1, 叶静1   

  1. 1. 安顺学院化学与生物农学系, 贵州, 安顺 550025;
    2. 贵州大学生命科学学院, 贵阳 550025
  • 收稿日期:2011-01-14 出版日期:2011-09-30 发布日期:2017-04-12
  • 基金资助:
    贵阳市科技基金项目

Study on Liquid Fermentation of Mucor mucedo (L.) Fres

LONG Ju1, WU Yong-jun2, HE Ying-xia1, YE Jing1   

  1. 1. Department of Chemistry and Bioagronomy, Anshun College, Anshun 561000, Guizhou, China;
    2. College of Life Science, Guizhou University, Guiyang 550025, China
  • Received:2011-01-14 Online:2011-09-30 Published:2017-04-12

摘要: 利用豆腐生产工艺中的副产物黄泔水为溶剂,通过单因素实验和正交实验,在28℃、200 r/min摇瓶培养条件下,确定高大毛霉MHC-7最佳培养基为黄豆粉3%、可溶性淀粉0.4%、硫酸镁0.2%、磷酸二氢钾0.2%、蔗糖0.3%和黄泔水。培养基中碳氮比为1.5、最适接种量为6%、最适起始pH值为6.0,通过生物量测定,MHC-7液体培养在36 h达到最大值(1.363 g/100 mL)。

关键词: 高大毛霉, 液体培养, 生长曲线

Abstract: Using yellow hogwash,the byproduct in the production of fermented tofu,as the solvent,via the single element and orthogonal experiment,the optimum medium of Mucor mucedo (L.) Fres is determined:shaking with 28℃,200 r/min,3% soybean powder,0.4% soluble starch,0.2% magnesium sulfate,0.2% potassium dihydrogen phosphate,0.3% sucrose and yellow hogwash.The adequate C:N ratio in medium is 1.5. The initial optimal inoculum is 6% and pH is 6.0. Through biomass determination,the fermentation in liquid reached the maximum value in the 36 hour(1.363 g/100 mL).

Key words: Mucor mucedo (L.) Fres, liquid fermentation, growth curve

中图分类号: 

  • TS261.15