四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (03): 386-390.doi: 10.3969/j.issn.1000-2650.2011.03.016

• • 上一篇    下一篇

暗孢节菱孢菌非蛋白类毒素的基本性质研究

李姝江, 朱天辉, 胡彬弘, 杨莉, 张丽娜   

  1. 四川农业大学林学院, 四川 雅安 625014
  • 收稿日期:2011-07-04 出版日期:2011-09-30 发布日期:2017-04-12
  • 通讯作者: 朱天辉(E-mail:zhutianhui@tom.com) E-mail:zhutianhui@tom.com
  • 基金资助:
    国家自然科学基金(31070578)

Basic Properties of the Nonprotein Toxin from Arthrinium phaeospermum

LI Shu-jiang, ZHU Tian-hui, HU Bin-hong, YANG Li, ZHANG Li-na   

  1. College of Forestry, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2011-07-04 Online:2011-09-30 Published:2017-04-12

摘要: 用改进的Fries培养基在最佳条件下培养出的暗孢节菱孢菌[Arthrinium phaeospermum(corda)M. B. Ellis]滤液,采用硫酸铵沉淀制备蛋白类、非蛋白类粗毒素。经致病性检测,前者生物活性低于后者小分子毒素成分。基本性质研究显示非蛋白粗毒素有一定专化性,但专化性不强,介于专化性与非专化性毒素之间;具有较强的热和紫外线稳定性、对pH敏感,易受碱性物质影响;9种不同极性物质对毒素的生物活性有不同影响,该毒素可能为极性较大的物质。

关键词: 毒素, 基本性质, 杂交竹, 专化性, 暗孢节菱孢菌

Abstract: The crude protein toxin and nonprotein toxin were prepared by the ammonium sulphate precipitation from the filtrate of A. phaeospermum cultured in the modified Fries culture medium under the optimum condition.The pathogenicity test showed the former had lower toxic activity than the latter with small molecule.Basic properties indicated that the nonprotein toxin had host-specificity to a certain degree,but not absolutely,and was situated between HST and NHST.Meanwhile,the nonprotein toxin had strong thermal and ultraviolet stabilities,was sensitive to pH,and easily affected by the alkalinity substance.Nine polar substances had different effects on the activity of toxin,which might be polar compound.

Key words: toxin, basic property, hybrid bamboo, specificity, Arthrinium phaeospermum

中图分类号: 

  • S718.81