四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (01): 40-44.doi: 10.3969/j.issn.1000-2650.2011.01.008

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基于ITS序列分析探讨大兴安岭地区野生黑木耳菌株的遗传多样性

刘华晶1,2, 许修宏1, 姜廷波2, 邹学忠2   

  1. 1. 东北农业大学资源与环境学院, 哈尔滨 150030;
    2. 东北林业大学林学院, 哈尔滨 150040
  • 收稿日期:2010-08-14 出版日期:2011-03-31 发布日期:2017-04-11
  • 通讯作者: 许修宏(E-mail:howard2857@hotmail.com) E-mail:howard2857@hotmail.com
  • 基金资助:
    科技部十一五科技支撑项目(2008BADA1B01);科技部十一五科技支撑项目(2008BAD95B06-03);东北农业大学资源与环境学院青年基金(ZHQJ2009-14);东北农业大学创新团队发展计划(CXT003-2-1)

A Study on Genetic Diversity of wild Auricularia auricula in Greater Khingan Mountains Area Based on ITS Sequence Analysis

LIU Hua-jing1,2, XU Xiu-hong1, JIANG Ting-bo2, ZOU Xue-zhong2   

  1. 1. Faculty of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China;
    2. Forestry Faculty, Northeast Forestry University, Harbin 150040, China
  • Received:2010-08-14 Online:2011-03-31 Published:2017-04-11

摘要: 通过ITS序列对大兴安岭地区采集的14株野生黑木耳菌株和6株对照栽培种黑木耳菌株进行亲缘关系分析,以揭示不同菌株之间的遗传关系。利用软件Clustal X 1.83和MAGA 4.1进行系统发育分析。结果表明:①黑木耳ITS区(ITS1+5.8S+ITS2)信息位点比例达到52.1%,遗传位点丰富,证明ITS序列可以为黑木耳菌株的亲缘关系问题提供较强的证据。②在MP系统树中,黑木耳菌株明显聚为2类,聚类结果表明黑木耳野生菌株间遗传多样性优于栽培菌株。

关键词: 黑木耳, ITS, 遗传多样性

Abstract: Genetic relationship among different strains was disclosed by analyzing the internal transcribed spacer(ITS) sequences of 14 wild Auricularia auricula strains and 6 cultivated strains from Greater Khingan Mountains area.The software ClustalX 1.83 and MEGA 4.1 were used to conduct the phylogeny analysis.The results were as follows:the information sites of ITS Region(ITS1+5.8S+ITS2) reached 52.1%, which proved that ITS sequence could provide strong evidences for the genetic relationship among Auricularia auricular strains.② in MP trees, Auricularia auricula strains were obviously divided into 2 groups, and there were more genetic diversities in wild strains than in cultivated strains.

Key words: Auricularia auricula, internal transcribed spacer sequence, genetic diversity

中图分类号: 

  • S646.6