四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (01): 16-21.doi: 10.3969/j.issn.1000-2650.2011.01.004

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苦荞转录因子基因FtMYB1的克隆及序列分析

白悦辰, 陶亮, 蒙华, 李成磊, 陈惠, 吴琦   

  1. 四川农业大学生命科学与理学院, 四川 雅安 625014
  • 收稿日期:2010-12-27 出版日期:2011-03-31 发布日期:2017-04-11
  • 通讯作者: 吴琦(E-mail:wuqiwq@yahoo.cn) E-mail:wuqiwq@yahoo.cn
  • 基金资助:
    四川农业大学"双支计划"

Clone and Sequence Analysis of a Transcription Factor Gene FtMYB1 from Fagopyrum tataricum

BAI Yue-chen, TAO Liang, MENG Hua, LI Cheng-lei, CHEN Hui, WU Qi   

  1. College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2010-12-27 Online:2011-03-31 Published:2017-04-11

摘要: 采用RACE-PCR技术,从苦荞花蕾克隆得到一个MYB基因(FtMYB1)。结果表明,FtMYB1基因DNA序列全长863bp(GenBank,JF313344),含两个内含子,内含子1长度为78bp(134~211bp),内含子2长度为74bp(342~415bp);cDNA序列全长711bp(GenBank,JF313345);开放阅读框(ORF)编码236个氨基酸,理论分子量26.9kDa,等电点6.33。序列比对及同源建模分析表明,FtMYB1蛋白与其他植物MYB结合域有高度的同源性,具有MYB同源蛋白的典型特征。

关键词: 苦荞, MYB转录因子, 基因克隆, 序列分析

Abstract: A transcription factor gene FtMYB1 was isolated from the flower buds of Fagopyrum tataricum by RACE-PCR method.The results showed that the full-length DNA of FtMYB1 was 863 bp(GenBank, JF313344) with two introns, 78 bp intron-1(134-211 bp) and 74 bp intron-2(342-415 bp).Meanwhile, the full-length cDNA of FtMYB1 was 711 bp(GenBank, JF313345).The open reading frame(ORF) of FtMYB1 encodes 236 amino acid residues with a predicted molecular mass of 26.9 kDa and an isoelectric point of 6.33.Sequence analysis and homology modeling have suggested that FtMYB1 shows high homology with other botanic MYB.

Key words: Fagopyrum tataricum, MYB transcription factor, gene cloning, sequence analysis

中图分类号: 

  • S517