四川农业大学学报 ›› 2016, Vol. 34 ›› Issue (04): 505-510.doi: 10.16036/j.issn.1000-2650.2016.04.019

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瘦素对鸡原代前体脂肪细胞增殖分化的影响

覃飞, 张羽, 刘嘉, 朱庆, 王彦, 尹华东, 李地艳, 赵小玲   

  1. 四川农业大学动物科技学院, 成都 611130
  • 收稿日期:2016-07-25 出版日期:2016-12-31 发布日期:2017-02-15
  • 通讯作者: 赵小玲,博士,教授,研究方向:家禽遗传育种与繁殖,E-mail:zhaoxiaoling04@163.com。 E-mail:zhaoxiaoling04@163.com
  • 作者简介:覃飞,农学学士。
  • 基金资助:
    国家自然科学基金项目(编号:31402070)

Effect of Leptin on the Proliferation and Differentiation of Chicken Primary Preadipocytes

QIN Fei, ZHANG Yu, LIU Jia, ZHU Qing, WANG Yan, YIN Hua-dong, LI Di-yan, ZHAO Xiao-ling   

  1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2016-07-25 Online:2016-12-31 Published:2017-02-15

摘要: [目的] 探究不同浓度瘦素对鸡原代前体脂肪细胞增殖分化的影响。[方法] 以12日龄科宝肉鸡腹部脂肪为材料进行原代前体脂肪细胞培养,细胞铺板24 h后,试验组细胞以添加了0、50、200、800 ng/mL瘦素的培养基进行培养,每隔24 h换一次液,至72 h。采用CCK8法绘制对照组和瘦素处理组细胞的生长曲线,油红O提取比色法测定脂肪含量变化,Real-time PCR方法检测前体脂肪细胞分化过程中关键基因PPARγSREBP1c的表达。[结果] CCK8法与油红O提取比色法表明,0~800 ng/mL瘦素对鸡前体脂肪细胞的增殖分化无显著影响(P>0.05),Real-time PCR结果表明不同浓度的瘦素处理对前体脂肪细胞内脂肪分化关键基因PPARγSREBP1c的表达无显著影响(P>0.05)。[结论] 本试验证明0~800 ng/mL瘦素对鸡前脂肪细胞的增殖和分化无影响。

关键词: 鸡前体脂肪细胞, 瘦素, 原代培养, 增殖分化, PPARγ, SREBP1c

Abstract: [Objective] In order to explore the effects of leptin on the proliferation and differentiation of chicken primary preadipocytes. [Methods] Separated from 12-day-old Cobb broilers abdominal fat for cell culture, cells were treated with 0, 50, 200, 800 ng/mL leptin after being plated for 24 h, respectively. Culture liquids were changed per 24 h time interval until 72 h. Growth curves for the four experimental groups were draw via CCK-8 method. The determination of fat content was done via oil red O extraction method. Expressions of key genes PPARγ and SREBP1c during the differentiation of preadipocytes were detected via q-PCR. [Results] The results of CCK-8 method and oil red O extraction indicated that 0~800 ng/mL leptin had no significant effect on the proliferation and differentiation of chicken preadipocytes(P>0.05), meanwhile the Liptin had no significant effect on the expression of PPARγ and SREBP1c(P>0.05), respectively. [Conclusion] The study proved that leptin had no effect on the proliferation and differentiation of chicken preadipocytes.

Key words: chicken preadipocyte, Leptin, primary culture, proliferation and differentiation, PPARγ, SREBP1c

中图分类号: 

  • S831