四川农业大学学报 ›› 2016, Vol. 34 ›› Issue (04): 483-487.doi: 10.16036/j.issn.1000-2650.2016.04.015

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两株硫酸盐还原菌的培养条件研究

杜刚1, 张广求1, 胡秋月2, 郭沨3, 杨海英2   

  1. 1. 云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室, 昆明 650500;
    2. 云南民族大学化学与环境学院, 昆明 650500;
    3. 中国水电顾问集团公司, 北京 100011
  • 收稿日期:2016-06-29 出版日期:2016-12-31 发布日期:2017-02-15
  • 通讯作者: 杨海英,教授,主要从事微生物化学的研究,E-mail:yanghy200401@126.com。 E-mail:yanghy200401@126.com
  • 作者简介:杜刚,副教授,主要从事微生物研究,E-mail:dugang2006@aliyun.com。
  • 基金资助:
    国家自然科学基金资助项目(21462051);云南中烟工业有限责任公司项目(2015539200340277);云南省教育厅科学研究基金重点项目(2015Z111);云南民族大学民族药资源化学国家民委-教育部重点实验室开放基金项目(MZY1401)

Study on the Culture Conditions of Two Sulfate-Reducing Bacterium Strains

DU Gang1, ZHANG Guang-qiu1, HU Qiu-yue2, GUO Feng3, YANG Hai-ying2   

  1. 1. Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Kunming 650500, China;
    2. College of Chemistry and Environment, Yunnan Minzu University, Kunming 650500, China;
    3. The China Hydropower Consulting Group Corporation, Beijing 100011, China
  • Received:2016-06-29 Online:2016-12-31 Published:2017-02-15

摘要: [目的] 研究两株SRB菌生长繁殖的影响因素,进行SRB菌剂的初步试制。[方法] 采用CFU法进行菌株密度计数,考察不同pH、培养温度、碳源、培养基等条件对两株SRB菌株生长的影响,并对SRB4菌株进行了菌剂的初步试制。[结果] 菌株SRB4的最适培养条件:温度为38℃;起始pH为8.0;培养基是以柠檬酸钠为碳源的改良Postgate培养基。菌株SRB3的最适培养条件:温度为36℃;起始pH为7.0;培养基是以玉米芯水提液为碳源的Postgate培养基。经菌剂初步试制,SRB4菌株在发酵48 h后菌株密度保持在1012 CFU/mL以上。[结论] 两株SRB菌在最适培养条件下密度保持在1012 CFU/mL以上,可作为工程应用的备选菌株。

关键词: 硫酸盐还原菌, 培养条件, 菌剂

Abstract: [Objective] The main factors of the growth of two Sulfate-Reducing Bacterium(SRB) strains were investigated and preliminary trail of SRB microbial inoculum was carried out. [Methods] The impact of pH, temperature, carbon source and culture media on the growth of SRB were studied by the method of CFU for strain density count, moreover, the trial-produce of SRB4 microbial inoculum was implemented. [Results] The optimum culture conditions for SRB4 were as follows:temperature 38℃; pH 8.0; provided Postgate medium with sodium citrate as carbon source. The optimum culture conditions for SRB3 were as follows:temperature 36℃; pH 7.0; Postgate medium with the water extract of corn cob as carbon source. The strain density of SRB4 was maintained above 1012 CFU/mL after 48 h fermentation. [Conclusion] Under the optimal conditions, the strain density of the two SRBs were up to 1012 CFU/mL, and they could be alternative strains for engineering application.

Key words: sulfate reducing bacteria, culture conditions, microbial inoculum

中图分类号: 

  • X703