四川农业大学学报 ›› 2016, Vol. 34 ›› Issue (04): 445-449.doi: 10.16036/j.issn.1000-2650.2016.04.009

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珍稀濒危植物宝华玉兰遗传多样性的ISSR分析

陈云霞, 南程慧   

  1. 南京森林警察学院, 南京 210023
  • 收稿日期:2016-09-01 出版日期:2016-12-31 发布日期:2017-02-15
  • 作者简介:陈云霞,博士,主要从事野生动植物鉴定与保护研究,E-mail:yunaini@163.com。
  • 基金资助:
    中央高校基本科研业务费专项资金项目(LGZD201505)

ISSR Analysis on Genetic Diversity of Endangered Plant Magnolia zenii

CHEN Yun-xia, NAN Cheng-hui   

  1. Nanjing Forest Police College, Nanjing 210023, China
  • Received:2016-09-01 Online:2016-12-31 Published:2017-02-15

摘要: [目的] 通过ISSR分子标记技术,分析宝华玉兰种质资源的遗传多样性,为该珍稀濒危植物的利用和保护提供理论依据和技术支持。[方法] 利用ISSR分子标记对江苏句容宝华山的20个宝华玉兰单株进行遗传多样性分析。[结果] 用筛选获得的6条引物对样品进行PCR扩增,共扩增出50个清晰的扩增位点,其中多态性位点34个,多态性位点比率为(PPB)68%。通过POPGENE32软件计算出有效等位基因数(Ne)、Nei's基因多样性指数(H)和Shannon信息多样性指数(I)分别为1.4068、0.2356、0.3512。利用NTSYSpc-2.1软件进行UPGMA聚类,各个样品间的遗传相似系数在0.58~1.0。[结论] 宝华玉兰具有丰富的遗传多样性,20个野生单株间的遗传相似度较高,亲缘关系较近。

关键词: 宝华玉兰, ISSR, 遗传多样性, 保护

Abstract: [Objective] The aim of the study was to assess the genetic diversity of Magnolia zenii by using inter-simple sequence repeat(ISSR)markers technique. The results could provide a theoretical basis and technical support for the use and protection of this rare and endangered species. [Methods] ISSR markers was used to assess the genetic diversity of 20 Magnolia zenii individuals. [Results] Six primers were selected and used for PCR amplification. 50 discernible DNA fragments were produced. of these, 34 were polymorphic loci and the percentage of polymorphic loci PPB was 68%. Based on POPGENE32, the effective number of alleles(Ne), Nei's gene diversity(H) and Shannon's Information index(I) were 1.406 8, 0.235 6 and 0.351 2, respectively. According to cluster analysis with NTSYSpc-2.1, genetic similarity coefficient between each sample ranged from 0.58 to 1.0. [Conclusion] There is abundant genetic diversity of Magnolia zenii and there is high genetic similarity and close genetic relationship among the 20 individuals.

Key words: Magnoliazenii, ISSR, genetic diversity, protection

中图分类号: 

  • Q16