关键词: 菊花, 基因, 克隆, 表达载体

Abstract: [Objective] The gene of CmLhcb1 was cloned and transferred into Arabidopsis. The function of the gene was explored. [Method] Chrysanthemum named ‘Puma sunny’ seeding was used as experimental material. We use PCR (Polymerase Chain Reaction)method in vitro to amplify chlorophyll a/b binding protein gene (CmLhcb1). The plant expression vector of chrysanthemum pEarleyGate103-CmLhcb1 was constructed. We got 3 T3 positive transgenic Arabidopsis plants by agrobacterium mediated method. [Results] There was high similarity between the protein coding sequence of the gene and the those of other species. The major differences were in the former 50 amino acids of C terminal. The analysis of gene expression profile showed that the gene expression increased significantly under the conditions of 15% light and gibberellin (P<0.05). The expression was the greatest in leaves but was the lowest in root. The expression of day was 30 times higher than that of the evening. [Conclusion] The CmLhcb1 gene of C. morifolium had significant specific tissue expression when it was induced by light or gibberellin.

Key words: Chrysanthemum morifolium, gene, cloning, expression vector