四川农业大学学报 ›› 2015, Vol. 33 ›› Issue (01): 1-5.doi: 10.16036/j.issn.1000-2650.2015.01.001

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以色列二穗短柄草休眠及遗传多样性分析

黄青1, 薛文韬1, 严俊2, 赵钢2, 程剑平1   

  1. 1. 贵州大学麦作研究中心, 贵阳 550025;
    2. 成都大学生物产业学院, 成都 610106
  • 收稿日期:2014-12-15 出版日期:2015-03-31 发布日期:2017-02-28
  • 通讯作者: 程剑平,博士,教授,从事麦类遗传育种研究,E-mail:chengjianping@gmail.com。 E-mail:chengjianping@gmail.com
  • 作者简介:黄青,学士。
  • 基金资助:
    贵州省国际科技合作计划项目(黔科合外G字(2012)7011);四川省国际科技合作与交流研究计划项目(12GH0093)

Seed Dormancy and Genetic Diversity of Brachypodium distachyon from Israe

HUANG Qing1, XUE Wen-tao1, YAN Jun2, ZHAO Gang2, CHENG Jian-ping1   

  1. 1. Institute of Triticeae Crops, Guizhou University, Guiyang 550025, China;
    2. Faculty of Biotechnology Industry, Chengdu University, Chengdu 610106, China
  • Received:2014-12-15 Online:2015-03-31 Published:2017-02-28

摘要: [目的] 为探讨以色列地区二穗短柄草的休眠及其遗传多样性,对来自以色列不同地区的13个基因型二穗短柄草进行籽粒休眠深度和EST-SSR标记遗传位点多态性分析。[方法] 休眠深度分别测定籽粒在40℃高温不同贮藏时间下的最大发芽率(Gmax),并对其Gmax进行动态方程拟合;SSR标记的扩增片段采用PEGA凝胶电泳分离并用银染法进行显影。[结果] 13个基因型中,EG5休眠最深,而基因型Ko12和SB2休眠较浅。SSR标记扩增片段的聚类分析中,Sha4与Bd群体的基因型遗传结构较类似。[结论] 以色列不同地区籽粒休眠深度差异较大;北部Shlomi地区的基因型与土耳其地区的二穗短柄草有较近的亲缘关系。

关键词: 二穗短柄草, 种子休眠, 多样性, 以色列

Abstract: [Objective] To estimate the seed dormancy and its genetic diversity of B. distachyonin Israel, B. distachyon of 13 genotypes from different areas of Israel were tested for their dormancy depth and EST-SSR diversity. [Method] In the dormancy measurements, the maximum germination rate (Gmax)was measured and the equation of Gmax dynamic was fitted. The fragments amplified by SSR were separated by PEGA gel with develop method of silver staining. [Results] Among the 13 genotypes, seed dormancy of EG5 was the deepest but the seeds of Ko12 and SB2 germinated faster compared to the others. The cluster results of SSR amplified fragments showed that there was similar genetic structure between Bd and Sha4 population. [Conclusion] Large differences were found in seed dormancy among the different genotypes in Israel. The genotype from Shlomi of north area of Israel was related to the B. distachyon populations from Turkey.

Key words: Brachypodium distachyon, seed dormancy, genetic diversity, Israel

中图分类号: 

  • Q945.35