四川农业大学学报 ›› 2007, Vol. 25 ›› Issue (04): 462-467.doi: 10.16036/j.issn.1000-2650.2007.04.024

• 研究论文 • 上一篇    下一篇

FQ-PCR检测肌注小鹅瘟病毒VP3基因疫苗在雏鹅体内的动态分布

黎敏1, 程安春1,2, 汪铭书1,2, 韩新锋1, 刘晓东1, 卢菲1, 车茜1, 陈孝跃1   

  1. 1. 四川农业大学 动物医学院禽病防治中心, 四川 雅安 625014;
    2. 四川农业大学 动物疫病与人类健康四川省重点实验室, 四川 雅安 625014
  • 收稿日期:2007-03-16 出版日期:2007-12-31 发布日期:2017-03-04
  • 基金资助:
    国家科技攻关重大项目(2004BA901A03);教育部"新世纪优秀人才支持计划"项目(NCET-04-0906);四川省重大基础研究项目(05JY029-109);四川省重点建设学科项目(SZD0418)

Dynamic Distribution of GPV-VP3 Gene Vaccine in Geese via Vaccinated with Intramuscular Injection

LI Min1, CHENG An-chun1,2, WANG Ming-shu1,2, Han Xin-feng1, LU Fei1, CHE Qian1, LIU Xiao-dong1, Chen Xiao-yue1   

  1. 1. Avain Disease Research Center, College of Veterinary Medicine, Sichuan Agriculture University, Yaan 625014, Sichuan, China;
    2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Yaan 625014, Sichuan, China
  • Received:2007-03-16 Online:2007-12-31 Published:2017-03-04

摘要: 将30日龄四川白鹅分别肌肉注射接种50μg、100μg和200μg的GPV-VP3基因疫苗(pcDNA-GPV-VP3),以生理盐水和空载体质粒pcDNA3.1(+)为对照,于免疫接种后1h、12h、1d、3d、7d、21d、35d、63d、105d和217d采集全血以及各组织器官,用实时荧光定量PCR(FQ-PCR)检测pcDNA-GPV-VP3在雏鹅体内的动态分布。结果表明:①pcDNA-GPV-VP3各剂量免疫雏鹅1h即可在各组织中被检测到,其中注射部位含量最高,肝、淋巴器官(脾、法氏囊、胸腺、哈氏腺)含量较高;②到217d时,50μg组免疫雏鹅各个组织器官内仍检测到pcDNA-GPV-VP3的存在,但多数组织器官中的含量比1h时约少了103~104;③血液中pcDNA-GPV-VP3的含量较少,且1h~217d各时间点的差异不显著(P≥0.05);④不同剂量pcDNA-GPV-VP3免疫雏鹅各组织中的含量呈现的总体规律为200μg组 > 100μg组 > 50μg组,但差异不显著(P≥0.05)。因此,pcDNA-GPV-VP3免疫雏鹅后1h时可分布至雏鹅体内各组织器官中并持续存在217d以上。

关键词: FQ-PCR, pcDNA-GPV-VP3, 肌肉注射, 雏鹅, 动态分布

Abstract: This paper concentrates on 30-day-old geese were administered with 50 μg, 100 μg, 200 μg of pcDNA-GPV-VP3 respectively by the route of intramuscular injection with Normal saline, pcDNA3.1(+) as control. To detect the recombinant plasmid in the samples of peripheral blood and inoculated muscle, heart, liver, spleen, lung, kidney, brain, thymus, gland, harderian gland, bursa of fabricius, pancreas, duodenum, jejunum, ileum, rectum, appendices and serum with FQ-PCR at the interval of 1 h, 12 h, 1 d, 3 d, 7 d, 21 d, 35 d, 63 d, 105 d and 217 d after administration. Results showed that:①The pcDNA-GPV-VP3 could be detected in all tissues and peripheral blood at 1 h post infection. The copy number was the cargese at the skin of injected spot, then come liver, and lymphatic organ (spleen, fabricius, thymus, gland of harder);②At 217 d post administration, pcDNA-GPV-VP3 could still be detected in tissues of 50 μg dose group. But the copy number decreased about 103~104 copies in the most tissues than it was at 1 h post administration;③The changement of the copy numbers in the serum was always small and not obvious with the time (P≥0.05);④The quantity of pcDNA-GPV-VP3detected in the experimental groups showed the relations with the quantity of the inoculation doses, but not proportion (P≥0.05). The results demonstrated that pcDNA-GPV-VP3 could distribute in all tissues of geese at at 1 h post infection and existed in those after 217 d post infection.

Key words: FQ-PCR, pcDNA-GPV-VP3, intramuscular, geese, dynamic distribution

中图分类号: 

  • S858.335