四川农业大学学报 ›› 2007, Vol. 25 ›› Issue (03): 328-331.doi: 10.16036/j.issn.1000-2650.2007.03.025

• 研究论文 • 上一篇    下一篇

用新型分子模版吸附树脂从发酵液中分离红霉素的研究

鲁力1, 王亚宁2, 熊春华3, 余蓉1, 王槐三2   

  1. 1. 四川大学 华西药学院, 成都 610065;
    2. 四川大学 国家生物医学材料工程中心, 成都 610065;
    3. 浙江工商大学 化学系, 杭州 330306
  • 收稿日期:2006-10-12 出版日期:2007-09-30 发布日期:2017-03-04
  • 基金资助:
    科技部科技型中小企业创新基金(06C26215100476);浙江省科技厅科技计划资助项目(2005C33043)。

Study on the Separation of Erythromycin from Fermentation Liquid by New Absorption Resin

LU Li1, WANG Ya-ning2, XIONG Chun-hua3, YU Rong1, WANG Huai-san2   

  1. 1. West China School of Pharmacy Sichuan University, Chengdu 610065, China;
    2. National Engineering Research Center for Biomaterials Sichuan University, Chengdu 610065, China;
    3. Department of Chemistry, Zhejiang Gongshang University, Hangzhou 310035, China
  • Received:2006-10-12 Online:2007-09-30 Published:2017-03-04

摘要: 采用新型分子模版吸附树脂动态吸附、丙酮解吸、水稀释结晶和丙酮重结晶等流程,从红色链霉菌发酵滤清液中分离红霉素,研究影响吸附分离效率的各种因素,并与现行醋酸丁酯萃取生产流程比较。结果显示,改性LX-18树脂对红霉素的吸附量达130 ku/mL,对发酵液中色素的选择分离率>95%,红霉素浓度120~150 ku/mL的丙酮解吸液采用水稀释结晶,再用丙酮重结晶,即获得各项理化指标达药典标准的红霉素。结晶母液须2次吸附回收,红霉素总收率为60%~62%。本工艺具有简便、分离成本较低和减轻废水污染的特点。

关键词: 红霉素, 树脂吸附, 发酵液

Abstract: Useing neotype resin development-absorbing, acetone-desorption, water diluting to crystallize and acetone-recrystallisation separate the Erythromycin from red streptomycete fermentation scrubber liquid. Study all kinds of influence facto rs and com pare with currently butylacetate-abstraction production flow-sheet. The result shows that the new reshaping resin LX-18 does so well in absorbing the Eryhromycin, its adsorption quantity reach 130 ku/m L and the separation for pigmentosus > 95%, the Erythromycin concentration by 120~150 ku/m L of acetone-desorption liquid use water diluting to crystallize, then acetone-recrystallisation. After that, it's all physical and chemical indicatrix can gain the pharmacopoeia standard. The crystal mother liquid need to twice adsorbing-recover, and the total yield of Erythromycin can reach 60%~62%. This separation method possesses simple process, high efficiency, low cost and only mild pollution wastewater.

Key words: Erythromycin, absorption resin, fermentation liquid

中图分类号: 

  • TQ465.5