关键词: 芽孢杆菌, 纤维素酶, 筛选, 鉴定

Abstract: One strain of cellulase-producing bacteria which can degrade sodium carboxymethylcellulose was isolated on the selected medium by using CMC-Na as only carbon source from the mud. The strain has high endo-β-glucanase producing ability proved by the congo red dying method on the culture plates. It is identified as Bacillus subilius according to its morphological, physiological and biochemiscal characteristics. It is revealed by the experiments in which C-36 grows in a broad range of pH. While the optimum pH for its growing is pH 7.0 and it also grows well in more alkaline circumstances. Results in shaking flask culture show that high cellulase activity(CMCase) can be detected in the condition of pH 7.0 and the cellulase activity is 1.280 IU/mL.

Key words: bacillus, cellulose, selection, identification