四川农业大学学报 ›› 2004, Vol. 22 ›› Issue (02): 107-111.doi: 10.16036/j.issn.1000-2650.2004.02.004

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西藏小麦醇溶蛋白Gli-1和Gli-2位点等位基因组成分析

代寿芬, 郑有良, 颜泽洪   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2004-03-30 出版日期:2004-06-30 发布日期:2017-03-04
  • 基金资助:
    国家自然科学基金项目(30370882);四川省教育厅项目;四川省科技厅项目

Analysis of Gliadin Allele Compositions at Gli-1 and Gli-2 Loci in Tibet Wheat(Triticum aestivum)

DAI Shou-fen, ZHENG You-liang, YAN Ze-hong   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2004-03-30 Online:2004-06-30 Published:2017-03-04

摘要: 利用A-PAGE方法,鉴定了130份西藏小麦地方品种的Gli-1和Gli-2位点等位基因组成。结果表明,西藏小麦的6个主要醇溶蛋白位点等位基因组成类型丰富。共出现了98个等位基因,114种组合。各位点平均Nei氏遗传变异系数高达0854。在各位点基因中,Nei氏遗传变异系数在第一和第六同源群的相应位点表现相同趋势,以A组最高,B组次之,D组最低。6个主要醇溶蛋白位点的等位变异7~24个,平均1633个。出现频率最高的分别是Gli-A1urt3(14.62%)、Gli-B1k(15.38%)、Gli-D1a(40.00%)、Gli-A2o(14.62%)、Gli-B2a(22.38%)和Gli-D2a(40.00%)。在Gli-A1、Gli-B1和Gli-A2位点上分别出现了4种、2种和2种前人未报道的遗传块。在Gli-B1位点,有3份材料未表达。

关键词: 西藏小麦, 地方品种, 醇溶蛋白, 等位基因, A-PAGE

Abstract: Tibet wheat landraces were investigated for their gliadin allele compositions by acidic polyacrylamide gel electrophoresis(A-PAGE). The results suggest that there is high variance among six major loci of gliadin in Tibet wheat. There are 98 types of alleles and 114 gliadin combinations. The average Nei's genetic variation index(H) of six main loci is up to 0.854. The genetic variation indexes of the first and the sixth group chromosomes have the similar trend. A's is the highest, B's is the secondary and D's is the lowest. The allele types in six main gliadin loci vary from 7 to 24, with average of 16.33. The highest frequency at loci in Gli-A1, Gli-B1, Gli-D1, Gli-A2, Gli-B2 and Gli-D2 is urt3(14.62%), k(15.38%), a(40.00%), o(14.62%), a(22.38%) and a(40.00%), respectively. There are 4, 2 and 2 kinds of gliadin blocks in each of the Gli-A1, Gli-B1 and Gli-A2 loci have not been characterized. At the Gli-B1 loci, three Tibet wheat landrace can not encode any expressed gliadin band.

Key words: Tibet wheat, landrace, gliadin, allele, A-PAGE

中图分类号: 

  • S512.1