四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (04): 398-399,410.doi: 10.16036/j.issn.1000-2650.2001.04.022

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小麦新品种R59成熟胚的组织培养初报

唐宗祥, 任正隆, 张怀琼, 张怀渝, 傅体华   

  1. 四川农业大学 植物遗传育种省级重点实验室, 四川 雅安 625014
  • 收稿日期:2001-09-01 出版日期:2001-12-31 发布日期:2017-03-06
  • 基金资助:
    国家自然科学基金项目(39893350)

Primary Study on Callus Culture in Mature Embryo of New Wheat Varieties R59

TANG Zong-xiang, REN Zheng-long, ZHANG Huai-qiong, ZHANG Huai-yu, FU Ti-hua   

  1. State Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2001-09-01 Online:2001-12-31 Published:2017-03-06

摘要: 选配3种培养基,以小麦新品种R59的成熟胚作外植体进行组织培养。结果显示:不同培养基中的成愈情况差异较大,其中培养基Ms+2,4-D2.0mg/l+KT0.5mg/L的效果较好,成愈率高达92%~93.3%。结果同时表明:胚芽对形成胚性愈伤组织不利。

关键词: 小麦, 成熟胚, 组织培养

Abstract: Mature embryos of new wheat variety R59 were used as explant for culture in vitro in three kinds of culture medium. The result shows that the ratio of callus induced is greatly different and the ratio of callus induced from the better culture medium(Ms+2, 4-D2.0mg/L+KT0.5mg/L) is 92%~93.3%. The result also shows that embryonic bud is disadvantageous to inducing embryonic callus.

Key words: WHEAT, MATURE EMBRYO, CALLUS CULTURE

中图分类号: 

  • S512.1