四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (04): 394-397.doi: 10.16036/j.issn.1000-2650.2001.04.021

• • 上一篇    下一篇

几个高抗条锈病的优质四川小麦品系选育研究

舒焕麟1, 杨足君1, 李光蓉1, 任正隆2, 朱华中3   

  1. 1. 四川农业大学 小麦研究所, 四川 都江堰 611830;
    2. 四川农业大学 植物遗传育种省级重点实验室, 四川 雅安 625014;
    3. 四川省农科院作物所, 四川 成都 610066
  • 收稿日期:2001-09-01 出版日期:2001-12-31 发布日期:2017-03-06
  • 基金资助:
    四川省教育厅重点科研资助项目

Improving End-use Quality of Several Wheat Lines with High Resistance to Stripe Rust

SHU Huan-lin1, YANG Zu-jun1, LI Guang-rong1, REN Zheng-long2, ZHU Hua-zhong3   

  1. 1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City 611830, Sichuan, China;
    2. State Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    3. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
  • Received:2001-09-01 Online:2001-12-31 Published:2017-03-06

摘要: 运用A-PAGE及SDS-PAGE技术,对6个杂交组合入选的F4~F8世代的高抗条锈病株系进行品质优化筛选。从140个高抗条锈病株系中筛除了42个(30%)具有Gli-B1l位点的1RS/1BL易位系;在89个无1RS/1BL染色体的株系中,选出了32个(35.96%)HMW-GS组成为1或2*或N(Glu-A1)、7+8或17+18(Glu-B1)、5+10(Glu-D1)株系;SDS沉淀值采用CIMMYT微量法测试,受检9个材料中的4个达到了12.0~14.5mL。结果证明,在可以鉴别杂种后代抗条锈性的世代才开始运用A-PAGE和SDS-PAGE技术进行品质性状选择,是提高四川小麦抗病及品质育种选择效率的方法。

关键词: 小麦, 抗条锈性, 醇溶蛋白, 谷蛋白, SDS沉淀值

Abstract: With aim to optimize the end use quality of wheat lines in breeding program, total 140 F4 to F6 progenies exhibiting high resistance to stripe rust derived from 6 crosses were examined by APAGE and SDS-PAGE. 42 progenies(about 30%) contained 1RS/1BL wheat rye translocation chromosome which expresses the gliadin encoded by Gli-B1l. From the 89 non 1RS/1BL lines, we selected 32 progenies(about 35.96%) with HMW-GS composition of 1, 2* or N in Glu-A1, 7+8 or 17+18 in Glu-B1, 2+12 or 5+10 in Glu-D1.4 in the above 9 selected lines embodied SDS sediment value up to 12.0~14.5mL by small amount measurement, which referred the protocol from CIMMYT. The result indicates that applying the APAGE and SDS-PAGE to select the progenies with high resistance to stripe rust is a reliable method to enhance selective efficiency for combining rust resistance and quality improvement in Sichuan wheat breeding practice.

Key words: WHEAT, STRIPE RUST RESISTANCE, GLIADIN, GLUTENIN, SDS SEDIMENT VALUE

中图分类号: 

  • S512.034