四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (04): 380-383.doi: 10.16036/j.issn.1000-2650.2001.04.018

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我国部分小麦新品种(系)的高分子谷蛋白亚基遗传变异分析

晏本菊1, 任正隆2   

  1. 1. 四川农业大学 基础部, 四川 雅安 625014;
    2. 四川农业大学 植物遗传育种省级重点实验室, 四川 雅安 625014
  • 收稿日期:2001-09-01 出版日期:2001-12-31 发布日期:2017-03-06
  • 基金资助:
    四川省科技厅应用基础研究项目资助

Allelic Variation of Glu-1 in Chinese Some Wheat Varieties or Line

YAN Ben-ju1, REN Zheng-long2   

  1. 1. Department of Basic Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2001-09-01 Online:2001-12-31 Published:2017-03-06

摘要: 利用SDS-PAGE方法对我国52份新育成的优质品种(系)的高分子谷蛋白亚基进行了分析。按照Payne的谷蛋白亚基评分标准进行了品质评分。结果表明,这些品种(系)的品质评分为7.28,高分子谷蛋白变异较为丰富,Glu-A1位有两个等位变异"N"和"1"主要为1亚基,占(73.1%);Glu-B1有7+8(46.3%)、7+9(36.5%)、20(5.8%)、17+18(3.8%)、13+16(3.8%)、14+15(3.8%)6个等位变异类型,但主要以7+8和7+9为主;Glu-D1有5+10(36.6%)、2+12(51.9%)、4+12(11.5%)3个等位变异类型,以2+12和5+10为主。其结果基本反映了我国目前培育的小麦品种的谷蛋白亚基组成情况。研究还证明优质亚基5+10、2*亚基在我国小麦品种中的比例偏低,因此在育种中应加强优质的谷蛋白亲本材料的引进和利用,并对材料中的优质基因源在四川小麦优质育种中的应用进行了讨论。

关键词: 小麦品种, 谷蛋白亚基, 品质

Abstract: The high-molecular weight subunits of Wheat Glutenin in 52 Wheat Varieties(line) were fractionated by SDS-PAGE. The quality scores of genotypes range from 4~10. The mean score in these varieties(line) is 7.28. The frequencies of HMW-GS alleles of the 52 Varieties(line) are:Two types were detected on Glu-A1 loci, which are nll(c26.9%), 1(a73.1%). Six types were detected on Glu-B1 loci, which are 7+8(b46.3%), 7+9(c36.5%), 20(5.8%), 17+18(i3.8%), 13+16(3.8%), 14+15(h3.8%). 7+8 and 7+9 were two major types. Three types were detected on Glu-D1 loci, which are 2+12(a51.9%), 5+10(d36.6%), 4+12(c11.5%). 2+12 and 5+10 are two major types. The result basically reflects allelic changes on Glu-1 in Chinese Wheat varieties(lines) in nearly years. It proves in the study the frequency HMW-GS 2* and 5+10 is lower in the above Wheat varieties(line). Therefore, it is important that the good quality HMW-GS is introduced and applied. The wide application of this good quality gene in plant breeding in Sichuan is also discussed.

Key words: WHEAT VARIETIES, QUALITY, GLUTENIN SUBUNITS

中图分类号: 

  • S512.1.032