关键词: 杂细胞, 里氏木霉, 纤维素酶

Abstract: Cellulase production was carried out by solid state fermentation with Trichoderma reesei when the Parenchyma cell was used for inducers. The results show that the optimum conditions of producing cellulase are:the proportion of parenchyma cell to straw to wheat bran is 2:4:4, the nitrogen is(NH₄)₂SO₄, the water amount of medium is 200%, the temperature is 30℃ and the time is 5 days, in the solid state medium of parenchyma cell, straw powder and wheat bran. The CMCase is as high as 516.64IU/g dry koji. Tween 80 gives a little contribution to CMC ase. The optimal conditions for enzyme action are:pH4.85, 50℃.

Key words: PARENCHYMA CELL, TRICHODERMA REESEI, CELLULASE