四川农业大学学报 ›› 2000, Vol. 18 ›› Issue (03): 193-196.doi: 10.16036/j.issn.1000-2650.2000.03.001

• 研究快报 •    下一篇

簇毛麦抗白粉病基因向四川小麦转移的分子标记育种研究

杨足君1, 李光蓉1, 任正隆2   

  1. 1. 四川农业大学 小麦研究所, 四川 都江堰 611830;
    2. 四川农业大学 植物遗传育种省级重点实验室, 四川 雅安 625014
  • 收稿日期:2000-06-20 出版日期:2000-09-30 发布日期:2017-04-21
  • 基金资助:
    国家自然科学基金(批准号39700077)和四川省科委应用基础研究项目。

Molecular Marker assisted Selection in Transferring Powdery Mildew Resistance Gene from Dasypyrum Villosum to Sichuan Wheat

YANG Zu-jun1, LI Guang-rong1, REN Zheng-long2   

  1. 1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China;
    2. State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2000-06-20 Online:2000-09-30 Published:2017-04-21

摘要: 利用小麦6VS/6AL易位系的抗白粉病基因Pm21的SCAR标记,对不同簇毛麦来源的小麦抗白粉病材料及其杂交后代进行了分子鉴定和标记辅助选择研究,结果表明利用该标记可以鉴定小麦遗传背景下的簇毛麦6VS染色体臂,而且引物对小麦背景中的多年生簇毛麦也同样得到扩增。对簇毛麦染色体易位系6VS/6DL与四川小麦品种杂交的后代群体,可以利用SCAR标记进行辅助抗性选择。本文还对四川小麦分子标记辅助育种的策略进行了讨论。

关键词: 簇毛麦, 抗白粉病基因, 标记辅助选择, 四川小麦

Abstract: Molecular identification and marker assisted selection of wheat powdery mildew resistant lines with different originated Dasypyrum villosum and their progenies were carried out, by using the SCAR marker of Pm21 develop from a 6VS/6AL wheat-Dasypyrum translocation chromosomes line. The results are showed as following:1.The marker can easily detect the chromosome 6V of D. villosum in wheat background, and that of D. breviaristatum. It is likely that the SCARs can serve as specific markers to trace the corresponding chromatin of genus Dasypyrum; 2.The SCARs amplified from the progenies of the cross between pm34, a 6VS/6DL translocation line with Sichuan cultivated lines, can be used to distinguish the resistant plant from the breeding population. The utilization of the SCARs to wheat breeding for powdery mildew resistance is reliable and practical. In addition, the strategies of molecular marker-assisted selection in Sichuan wheat breeding are also discussed in the paper.

Key words: DASYPYRUM, POWDERY MILDEW RESISTANCE, MARKER ASSISTED SELECTION, SICHUAN WHEAT BREEDING

中图分类号: 

  • S512.1