四川农业大学学报 ›› 1996, Vol. 14 ›› Issue (04): 613-615,580.doi: 10.16036/j.issn.1000-2650.1996.04.027

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植物激素提纯中去除色素的有效方法研究

杨世民1, 何科学2, 赵彤3   

  1. 1. 四川农业大学农学院, 雅安市 625014;
    2. 江北县果树站园艺场, 江北县 620020;
    3. 西昌市公安局, 西昌市 615000
  • 收稿日期:1996-01-08 出版日期:1996-12-31 发布日期:2017-03-07

STUDY ON THE EFFICIENT METHOS OF ELIMINATING THE CHROMEONS IN ISOLATION AND PURIFICATION OF PHYTOHORMONES

Yang Shimin1, et al2   

  1. College of Agriculture, Sichuan Agricultural University/Yaan, Sichuan, China 625014
  • Received:1996-01-08 Online:1996-12-31 Published:2017-03-07

摘要: 用活性炭、聚乙烯吡咯烷酮(PVP)、C18胶柱作植物激素提纯的预处理,用高压液相色谱(HPLC)测定激素含量。研究结果表明:C18胶柱在三种方法中去除色素的效果最好,能从其处理的提取液中较准确地测定IAA、GAs、CTKs、ABA四种激素,回收率高;活性炭去除色素和酚类氧化物的效果也好,但对激素有较强的吸附作用;PVP的效果处于活性炭和C18胶柱之间。

关键词: 植物激素[45ND, 49LD], 活性炭[49K, 62D, 91B], 聚乙烯吡咯烷酮, C18胶柱, 高压液相色谱, 色素[45ND]

Abstract: Use actived carbon, PVP and Sep-Pak C18 Cartridge as pre-treaments in isolation and purification of phytohormones, dedermine the quantities of phytomones by HPLC. The research results as follow:The metod that treat with Sep-Pak C18 Cartridge is the best one in the three kinds of treatment. After the isolated liquids are treated with Sep-Pak C18 Cartridge, four kinds of phytohorm-ones(IAA, GA, CTK and ABA) can be determined accurately, and its phytohormones quantities have hight rate of retrieving. Although actived carbon can eliminate efficiently chromeons, but it can absorb a lot of phytohormones. The effect of treating with PVP is between acticed carbon and Sep-Pak C18 Cartridge.

Key words: PHYTOHORMONE, ACTIVED CARBON, PVP, SEP-PAK C18 CARTRIDGE, HPLC, PIGMENTS

中图分类号: 

  • Q946.885